DNA Microarray Technology

The DNA microarrays are manufactured using high speed robotics, generally on glass but sometimes on nylon substrates, for which probes of known identity are used to determine the routes, enabling the expression and massively parallel genetic studies and the discovery of genes. An experiment using a single DNA microarray can enable researchers to simultaneously obtain information on thousands of genes - a dramatic increase in performance compared to old methods involving the observation of the expression genetics.

The Microarray Facilities Located at the National Research Council Biotechnology Research Institute (NRC-BRI) of Montreal:

Portion of a Biochip:
The microarray facilities of the NRC-BRI in Montreal, was established in 2000 following an investment of $ 2 million of the IGS. It was one of the first laboratories of its kind in Canada and is recognized for its excellence.

A DNA chip or biochip consists of a microscope slide treated chemically to allow attachment of the DNA. DNA molecules are deposited in the form of very regular arrays of instruments with high precision robotic. Each deposit represents a different DNA fragment from a different gene. The chips are mainly used to study gene expression profiles for example between a normal cells and cancer cells where genes repressed cancer cells would be visualized by green dots, while the genes are turned red. Biochips are a very powerful screening that will distinguish between the genes responsible for the cancerous condition and those that are the consequence. This new technology now allows researchers to examine the interaction between all the genes of an organism at the same time instead of watching a few genes at once.

The NRC is a leader in the development of this new industry. Basic facilities include several elements, including bioinformatics, synthetic oligonucleides high performance, amplification, and stippling of biochips. DNA chips were provided to several programs of the intergenic spacer region (IGS), as well as universities and external partners and customers. The first chip Candida albicans (fungal pathogen) in the world was produced in these facilities.


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